Advanced Fluorescence Reporters in Chemistry and Biology II: by Ute Resch-Genger, Markus Grabolle, Roland Nitschke, Thomas

By Ute Resch-Genger, Markus Grabolle, Roland Nitschke, Thomas Nann (auth.), Alexander P. Demchenko (eds.)

From the reviews:

“This publication is a finished evaluation of the different sorts of nanoscale probes which have been and are at the moment being built for sensing of analytes. The well timed reference part permits the reader to find key references to the first literature for growth of the fabric lined. … a invaluable source that covers the artificial, photo-physical, and theoretical elements of this diversified box of research.” (Jeffrey T. Petty, magazine of the yankee Chemical Society, Vol. 133, 2011)

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By Ute Resch-Genger, Markus Grabolle, Roland Nitschke, Thomas Nann (auth.), Alexander P. Demchenko (eds.)

From the reviews:

“This publication is a finished evaluation of the different sorts of nanoscale probes which have been and are at the moment being built for sensing of analytes. The well timed reference part permits the reader to find key references to the first literature for growth of the fabric lined. … a invaluable source that covers the artificial, photo-physical, and theoretical elements of this diversified box of research.” (Jeffrey T. Petty, magazine of the yankee Chemical Society, Vol. 133, 2011)

Show description

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Additional info for Advanced Fluorescence Reporters in Chemistry and Biology II: Molecular Constructions, Polymers and Nanoparticles

Example text

Here, particulate labels and reporters are expected to have a bright future if the 32 U. Resch-Genger et al. nanotoxicity issue is resolved. There also exist many different instances where QDs have been applied to biological systems. Although most of these studies are proofof-principle, they underline the growing potential of these reagents. QDs are very attractive candidates for bioanalytical applications that can either exploit their potential for spectral multiplexing, do not require strong signal amplification or that rely on NIR fluorescence.

Sterically stabilized nanoparticles are mostly too large to enter cells, but are less likely to aggregate. A compromise can be reached by using smaller, but nevertheless still bulky, charged polyelectrolytes such as polyethyleneimine (PEI) [40], or an additional amphiphilic inorganic shell like silica [41, 42] which can be further functionalized using standard silica chemistry. It is difficult to predict the effect of surface functionalization on the optical properties of nanoparticles in general.

Thus, the search for brighter and especially more stable dyes is still going on. With respect to photochemical stability, lanthanide chelates can be superior to conventional organic chromophores. In contrast, almost all types of luminescent nanoparticles display excellent thermal and photochemical stability. From the range of these nanocrystals, QDs are the ones most sensitive to photooxidation and photobleaching, but even these effects can be almost completely suppressed by epitaxical growth of a protective shell to shield the core material for relevant time intervals [80].

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