Analyse 2: Exercises avec solutions by Ramis E., Deschamps C., Odoux J.

By Ramis E., Deschamps C., Odoux J.

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By Ramis E., Deschamps C., Odoux J.

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Reprinted from Electroencephalography and Clinical Neurophysiology, 38(1), Vertes RP, A device for recording single unit activity in freely-moving rats by a movable fine-wire microelectrode, 90–2, Copyright 1975, with permission from Elsevier. Reprinted from Physiology & Behavior, 19(3), Sinnamon HM, Woodward DJ, Microdrive and method for single unit recording in the active rat, 451–3 [100], Copyright 1977, with permission from Elsevier. Reproduced with permission from Ainsworth A, O’Keefe J. A lightweight microdrive for the simultaneous recording of several units in the awake, freely moving rat.

The basic concept is to have both the perfusion solution and the drug pumped through separate channels to a switch located on the animal’s head. Under these conditions, drugs could be delivered rapidly (within 1 min) of switching to the solution of interest. Optical Stimulation and Spike Recording The excitement for the optogenetic approach has become widespread. , channelrhodopsin-2) which have been expressed in specific neurons [93]. Activating these channels permits specific manipulations of the neurons at on the millisecond timescale [94].

J Neurosci. 1996;16(2):823–35. Epub 1996/01/15. 77. Lansink CS, Bakker M, Buster W, Lankelma J, van der Blom R, Westdorp R, et al. A split microdrive for simultaneous multi-electrode recordings from two brain areas in awake small animals. J Neurosci Methods. 2007;162(1–2):129–38. Epub 2007/02/20. 78. Kloosterman F, Davidson TJ, Gomperts SN, Layton SP, Hale G, Nguyen DP, et al. Microdrive array for chronic in vivo recording: drive fabrication. J Vis Exp. 2009. 3791/1094. Epub 2009/04/22. 79. Korshunov VA, Averkin RG.

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